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KMID : 0881720190340060519
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Journal of Food Hygiene and Safety
2019 Volume.34 No. 6 p.519 ~ p.525
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Analytical Method Development for Determination of Coenzyme Q10 by LC-MS/MS in Related Health Functional Foods
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Lee Jin-Hee
Oh Mi-Hyun
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Abstract
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The Ministry of Food and Drug Safety (MFDS) is amending its test methods for health functional foods (dietary food supplements) to establish regulatory standards and specifications in Korea. In this regard, we are continuing our research on analytical method development for the items listed in the Korean Health Functional Food Codex. In this study, we have developed a sensitive and selective test method that could simultaneously separate and determine coenzyme Q10 based on liquid chromatographic-tandem mass spectrometry (LC-MS/MS). Calibration curves showed linearity with a correlation coefficient (R2 ) of > 0.999 and the limits of detection (LODs) and limits of quantitation (LOQs) were in the range of 26.0 μg/L and 78.9 μg/L, respectively. The recovery results ranged between 98.6-107.0% at 3 different concentration levels with relative standard deviations (RSDs) less than 5%. The proposed analytical method was characterized with high resolution of the coenzyme Q10 and the assay was fully validated as well.
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KEYWORD
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Coenzyme Q10, Analytical method, LC-MS/MS, Health functional food
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